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By Leo A Goldblatt

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1962), de Iongh et al. (1962), and Lafont (1963) used the glucose-ammonium nitrate medium of Brian et al. (1961). , 1966). , 1963; Schroeder, 1966). Wildman et al. (1967) and Mateles and Wogan (1967) summarized much of the information available on aflatoxin production in nutrient solution. , 1966). B. e. stationary, shake or submerged culture. Codner et al. (1963) obtained yields of 100-200 mg/liter in 250-ml rotary shake flasks, whereas the same strain of the fungus did not produce any aflatoxins in 3- and 20-liter stirred aerated fermentors.

Observations on the relation of time to formation of aflatoxin in peanuts after digging have given varying results. Bampton (1963) pointed out that under tropical conditions (Africa), groundnuts that were free of toxin at digging contained detectable toxin in 48 hours. Also, kernels with testae damaged during shelling showed sporulating A. flavus in 4 days. McDonald and A'Brook (1963) found that 4-6 days in the field before artificial drying gave toxin-free kernels, but samples that had been left for 8-12 days or sun-dried for 10-16 days gave low to medium toxin yields (25-500 j^g/kg).

Flavus -NRRL 3145 A. flavus -NRRL 3145 591 363d 286d 1628 913 1963 680 1100 1100 2250 Codner et al. Vogel et al. Shotwell et al. Cucullu et al. Schroeder (1963) (1965) (1966) (1966) (1966) Substrate Boiler and (1966) Schroeder (1966) Nartey Diener and Davis (1966) Hesseltine et al. Eldridge Hesseltine et al. (1968a) (1967) Soybeans (1968a) Oatstraw Sweet clover, 376 105 30a oatstraw a Bi only. Germ or embryonic axis. c M-methionine. d Βχ + B 2 . b and other natural substrates has generally been established at substrate moistures in equilibrium with 70 % RH, at which very few fungi will grow.

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Aflatoxin by Leo A Goldblatt


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